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¹Ì»ý¹°¸í | Collinsella aerofaciens | Á¾·ù | T |
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KCCM : 12100, ATCC : 25986, IFO : , DSM : 3979, NRRL : | |||
¢¹ History (Eubacterium aerofaciens):<< ATCC << W.E.C. Moore VPI 1003. (ATCC 25986; DSM 3979). ¢¹ Ư¼º Human feces. Type strain(Skerman VB et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980; Moore WE et al. Eubacterium aerofaciens (Eggerth) Prevot 1938: Emendation of description and designation of the neotype strain. Int. J. Syst. Bacteriol. 21: 307-310, 1971; Kageyama A et al. Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. Nov., comb. Nov. Int. J. Syst. Bacteriol. 49: 557-565, 1999). Anaerobic. ¢¹ ¹è¾çÁ¤º¸ - CHOPPED MEAT MEDIUM Ground beef (free of fat), 500.0 gDistilled water, 1.0 L N NaOH, 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water, and NaOH and bring to a boil while stirring, Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1 liter original volume. To the filtrate add: Peptone, 30.0 g Yeast extract, 5.0 g K2HPO4, 5.0 g 0.025% Resazurin solution, 4.0 ml Boil, cool, and add 0.5g L-cysteine HCl H O, adjust pH 7.0. Under O -free nitrogen and 3% hydrogen, dispense 7 ml over 1 part meat particles to 4-5 parts fluid per test tube. Cap with rubber stoppers under N and H and autoclave in press for 15 minutes under fast exhaust. ¢¹ ¹è¾ç¿Âµµ : 37¡É ¢¹ °¡°ÝÄÚµå : 165,000¿ø ¢¹ ¿°±â¼¿ |