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검색 및 서비스 신청

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미생물 검색 리스트
미생물명 Collinsella aerofaciens 종류 T
KCCM : 12100, ATCC : 25986, IFO : , DSM : 3979, NRRL :

▷ History

(Eubacterium aerofaciens):<< ATCC << W.E.C. Moore VPI 1003. (ATCC 25986; DSM 3979).

▷ 특성

Human feces. Type strain(Skerman VB et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980; Moore WE et al. Eubacterium aerofaciens (Eggerth) Prevot 1938: Emendation of description and designation of the neotype strain. Int. J. Syst. Bacteriol. 21: 307-310, 1971; Kageyama A et al. Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. Nov., comb. Nov. Int. J. Syst. Bacteriol. 49: 557-565, 1999). Anaerobic.

▷ 배양정보 - CHOPPED MEAT MEDIUM

Ground beef (free of fat), 500.0 g
Distilled water, 1.0 L
N NaOH, 25.0 ml

Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water, and NaOH and bring to a boil while stirring, Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1 liter original volume. To the filtrate add:

Peptone, 30.0 g
Yeast extract, 5.0 g
K2HPO4, 5.0 g
0.025% Resazurin solution, 4.0 ml

Boil, cool, and add 0.5g L-cysteine HCl H O, adjust pH 7.0. Under O -free nitrogen and 3% hydrogen, dispense 7 ml over 1 part meat particles to 4-5 parts fluid per test tube. Cap with rubber stoppers under N and H and autoclave in press for 15 minutes under fast exhaust.

▷ 배양온도 : 37℃

▷ 가격코드 : 165,000원

▷ 염기서열

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